ATRAID Polyclonal antibody, PBS Only

ATRAID Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25548-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 25548-1-AP (same clone as 25548-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25548-1-AP (ATRAID antibody at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25548-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 25548-1-AP (same clone as 25548-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25548-1-AP (ATRAID antibody at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25548-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 25548-1-AP (same clone as 25548-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25548-1-AP (ATRAID antibody at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25548-1-PBS in a different storage buffer formulation.

---

Product Information

25548-1-PBS targets ATRAID in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATRAID fusion protein Ag22168
• Full Name: chromosome 2 open reading frame 28
• Calculated Molecular Weight: 229 aa, 25 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: BC035850
• Gene Symbol: ATRAID
• Gene ID (NCBI): 51374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATRAID, also named as APR3, p18, C2orf28, promotes osteoblast cell differentiation and terminal mineralization. It plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway. This antibody recognizes all the three isoforms (20-24 kDa, 16-19 kDa and 28-30 kDa).