ATP8A2 Polyclonal antibody, PBS Only

ATP8A2 Polyclonal Antibody for IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 31697-1-PBS

Synonyms

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Validation Data Gallery

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  IP experiment of mouse testis using 31697-1-AP (same clone as 31697-1-PBS)

  IP result of anti-ATP8A2 (IP:31697-1-AP, 4ug; Detection:31697-1-AP 1:600) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 31697-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 31697-1-AP (same clone as 31697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31697-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 31697-1-AP (same clone as 31697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31697-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 31697-1-AP (same clone as 31697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31697-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 31697-1-AP (same clone as 31697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31697-1-PBS in a different storage buffer formulation.

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Product Information

31697-1-PBS targets ATP8A2 in IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP8A2 fusion protein Ag36276
• Full Name: ATPase, aminophospholipid transporter-like, class I, type 8A, member 2
• Calculated Molecular Weight: 133kDa
• Observed Molecular Weight: 129 kDa
• GenBank Accession Number: NM_001313741
• Gene Symbol: ATP8A2
• Gene ID (NCBI): 51761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATP8A2 is also known as ATPIB. ATP8A2 is a member of the P4‐ATPase subfamily of P‐type ATPases that actively flips phosphatidylserine (PS) and phosphatidylethanolamine (PE) across membranes to generate and maintain transmembrane phospholipid asymmetry, a property important in such cellular processes as vesicle trafficking, apoptosis, cytokinesis, neuron survival, blood coagulation, and phagocytosis. ATP8A2 consists of an N‐domain involved in binding ATP, a P‐domain that undergoes phosphorylation, an A‐domain that functions in the dephosphorylation of the phosphorylated intermediate, and an M‐domain consisting of 10 transmembrane segments that comprise the pathway for translocation of the lipid across the membrane. ATP8A2 is highly expressed in the brain, spinal cord, testis, and retina (PMID: 33565221, PMID: 33766557, PMID: 37635783).