ATP8A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATP8A1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 21565-1-AP

Synonyms

ATP8A1, ATPase II, ATPASEII, ATPIA, ATPP2, Chromaffin granule ATPase II

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Validation Data Gallery

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  WB analysis of mouse brain using 21565-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21565-1-AP (ATP8A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21565-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21565-1-AP (ATP8A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IF: See 3 publications below

Product Information

21565-1-AP targets ATP8A1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP8A1 fusion protein Ag16071
• Full Name: ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
• Calculated Molecular Weight: 1164 aa, 131 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC109317
• Gene Symbol: ATP8A1
• Gene ID (NCBI): 10396
• RRID: AB_10734587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO J; Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase.; Authors - Shoken Lee
human	WB, IF	Proc Natl Acad Sci U S A; AP-3-dependent targeting of flippase ATP8A1 to lamellar bodies suppresses activation of YAP in alveolar epithelial type 2 cells.; Authors - Seunghyi Kook
mouse	WB	Sci Rep; Proteomic Analysis and Functional Characterization of P4-ATPase Phospholipid Flippases from Murine Tissues.; Authors - Jiao Wang
human	WB	iScience; Lipid flippase dysfunction as a therapeutic target for endosomal anomalies in Alzheimer's disease.; Authors - Nanaka Kaneshiro
human	WB	Clin Proteomics; Identification of potential molecular targets for the treatment of cluster 1 human pheochromocytoma and paraganglioma via comprehensive proteomic characterization; Authors - Ondrej Vit
rat	WB,IF	PLoS One; Proteomic Analysis of the Rat Canalicular Membrane Reveals Expression of a Complex System of P4-ATPases in Liver.; Authors - Pururawa Mayank Chaubey