ATP6V1H Monoclonal antibody

ATP6V1H Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G7B5

Cat no : 68425-1-Ig

Synonyms

ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13

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Validation Data Gallery

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  WB analysis using 68425-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68425-1-Ig (ATP6V1H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 68425-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68425-1-Ig (ATP6V1H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68425-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP6V1H antibody (68425-1-Ig, Clone: 3G7B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: U2OS cells, pig brain tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68425-1-Ig targets ATP6V1H in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP6V1H fusion protein Ag10130
• Full Name: ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
• Calculated Molecular Weight: 483 aa, 56 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC025275
• Gene Symbol: ATP6V1H
• Gene ID (NCBI): 51606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.