ATP6V1H Polyclonal antibody

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  WB analysis of mouse brain using 26683-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26683-1-AP (ATP6V1H Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 26683-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26683-1-AP (ATP6V1H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 26683-1-AP

  IP result of anti-ATP6V1H (IP:26683-1-AP, 4ug; Detection:26683-1-AP 1:300) with mouse brain tissue lysate 3000ug.

ATP6V1H Polyclonal Antibody for WB, IP, ELISA

Cat No. 26683-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IP: See 1 publications below

Product Information

26683-1-AP targets ATP6V1H in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6V1H fusion protein Ag24688
• Full Name: ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
• Calculated Molecular Weight: 483 aa, 56 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC025275
• Gene Symbol: ATP6V1H
• Gene ID (NCBI): 51606
• RRID: AB_2880601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UI12
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB	Int J Mol Sci; Atp6v1h Deficiency Blocks Bone Loss in Simulated Microgravity Mice through the Fos-Jun-Src-Integrin Pathway; Authors - Zanyan Zhao
human	WB	Front Immunol; Acidic Microenvironment Regulates the Severity of Hepatic Ischemia/Reperfusion Injury by Modulating the Generation and Function of Tregs via the PI3K-mTOR Pathway.; Authors - Xiaojie Gan
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human	WB	Biochem Biophys Res Commun; Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.; Authors - Jong-Jer Lee
rat	WB	Biophys Rep; PMID: 30596141; Authors - Min Li