ATP6V1G1 Polyclonal antibody

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  WB analysis of Jurkat using 16143-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16143-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16143-1-AP

  IP result of anti-ATP6V1G1 (IP:16143-1-AP, 4ug; Detection:16143-1-AP 1:500) with Jurkat cells lysate 3440 ug.

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  IHC staining of human kidney using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HEK-293 using 16143-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ATP6V1G1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16143-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

V-ATPase 13 kDa subunit 1, V ATPase subunit G 1, ATP6J, ATP6GL, ATP6G1

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Tested Applications

• Positive WB detected in: Jurkat cells, human kidney tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human kidney tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16143-1-AP targets ATP6V1G1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6V1G1 fusion protein Ag9217
• Full Name: ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1
• Calculated Molecular Weight: 118 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC008452
• Gene Symbol: ATP6V1G1
• Gene ID (NCBI): 9550
• RRID: AB_2062686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75348
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Traffic; Fast and cloning-free CRISPR/Cas9-mediated genomic editing in mammalian cells.; Authors - Paul T Manna
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human,mouse	WB,IHC	EBioMedicine; A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.; Authors - Irene Bertolini ; KD Validated
human	WB	EBioMedicine; Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo.; Authors - Andrea Terrasi
human	WB	Autophagy; Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate; Authors - Mengting Ou
mouse	WB	Cell; The proteomic landscape of synaptic diversity across brain regions and cell types; Authors - Marc van Oostrum