ATP6V1B2 Monoclonal antibody

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• KD/KO Validated

ATP6V1B2 Monoclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

3C11A9

Cat no : 68441-1-Ig

Synonyms

V-ATPase subunit B 2, VATB, HO57, ATP6B2, ATP6B1B2

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Validation Data Gallery

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  WB analysis using 68441-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68441-1-Ig (ATP6V1B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of A549 using 68441-1-Ig

  WB result of ATP6V1B2 antibody (68441-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected A549 cells.

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  IP experiment of HeLa using 68441-1-Ig

  IP result of anti-ATP6V1B2 (IP:68441-1-Ig, 4ug; Detection:68441-1-Ig 1:4000) with HeLa cells lysate 2000 ug.

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  FC experiment of HeLa using 68441-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug ATP6V1B2 Monoclonal antibody (68441-1-Ig, Clone:3C11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:40000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68441-1-Ig targets ATP6V1B2 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP6V1B2 fusion protein Ag7265
• Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 56-58 kDa
• GenBank Accession Number: BC003100
• Gene Symbol: ATP6V1B2
• Gene ID (NCBI): 526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).