ATP6V1B2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATP6V1B2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 15097-1-AP

Synonyms

ATP6B1B2, ATP6B2, ATP6V1B2, HO57, V ATPase subunit B 2, VATB, Vma2, VPP3

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Validation Data Gallery

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  WB analysis of mouse heart using 15097-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15097-1-AP

  WB result of ATP6V1B2 antibody (15097-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected HeLa cells.

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  WB analysis using 15097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15097-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15097-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15097-1-AP

  IP result of anti-ATP6V1B2 (IP:15097-1-AP, 4ug; Detection:15097-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 15097-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 15097-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15097-1-AP (ATP6V1B2 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, mouse heart tissue, mouse skeletal muscle tissue, mouse brain tissue, HeLa cells, rat brain tissue, K-562 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:40000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IP: See 1 publications below

Product Information

15097-1-AP targets ATP6V1B2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6V1B2 fusion protein Ag7174
• Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 56-58 kDa
• GenBank Accession Number: BC003100
• Gene Symbol: ATP6V1B2
• Gene ID (NCBI): 526
• RRID: AB_2243297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).

Publications

Species	Application	Title
human	WB	Nat Microbiol; The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.; Authors - Tomas Doyle
human	WB,IP	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
pig	WB	Microorganisms; Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.; Authors - Lanxin Yuan
human	WB	J Cancer; Upregulation of V-ATPase by STAT3 Activation Promotes Anoikis Resistance and Tumor Metastasis.; Authors - Funmilayo O Adeshakin
human	WB	Vet Res; Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.; Authors - Bingxin Chu