ATP6V1A Monoclonal antibody, PBS Only

ATP6V1A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C5G2

Cat no : 68440-1-PBS

Synonyms

ATP6A1, ATP6V1A, ATP6V1A1, HO68, V ATPase 69 kDa subunit, V ATPase subunit A, VA68, Vacuolar ATPase isoform VA68, Vma1, VPP2

---

Validation Data Gallery

• 

  WB analysis using 68440-1-Ig (same clone as 68440-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68440-1-Ig (ATP6V1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68440-1-Ig (same clone as 68440-1-PBS)

  WB result of ATP6V1A antibody (68440-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1A transfected HeLa cells. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68440-1-PBS targets ATP6V1A in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP6V1A fusion protein Ag10820
• Full Name: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• Calculated Molecular Weight: 617 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC013138
• Gene Symbol: ATP6V1A
• Gene ID (NCBI): 523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.