ATP6V1A Polyclonal antibody, PBS Only

ATP6V1A Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51152-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of HeLa using 51152-1-AP (same clone as 51152-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 51152-1-AP (same clone as 51152-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 51152-1-AP (same clone as 51152-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 51152-1-AP (same clone as 51152-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 51152-1-AP (same clone as 51152-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51152-1-AP (ATP6V1A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 51152-1-AP (same clone as 51152-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51152-1-AP (ATP6V1A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

---

Product Information

51152-1-PBS targets ATP6V1A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: NM_001690
• Gene Symbol: ATP6V1A
• Gene ID (NCBI): 523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.This antibody is specific to ATP6V1A.