ATP6V0A1 Polyclonal antibody

ATP6V0A1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13828-1-AP

Synonyms

V-ATPase 116 kDa subunit a 1, Stv1, Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, ATP6N1A, ATP6N1

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Validation Data Gallery

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  WB analysis using 13828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13828-1-AP (ATP6V0A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13828-1-AP

  IP result of anti-ATP6V0A1 (IP:13828-1-AP, 4ug; Detection:13828-1-AP 1:300) with mouse brain tissue lysate 3000ug.

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  IHC staining of mouse brain using 13828-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13828-1-AP (ATP6V0A1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 13828-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13828-1-AP (ATP6V0A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13828-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13828-1-AP (ATP6V0A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

13828-1-AP targets ATP6V0A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6V0A1 fusion protein Ag4191
• Full Name: ATPase, H+ transporting, lysosomal V0 subunit a1
• Calculated Molecular Weight: 837 aa, 96 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC032398
• Gene Symbol: ATP6V0A1
• Gene ID (NCBI): 535
• RRID: AB_2877979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation(PMID: 7774924 34904569).

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
human	WB,IP	Int J Biol Sci; Toosendanin, a novel potent vacuolar-type H+-translocating ATPase inhibitor, sensitizes cancer cells to chemotherapy by blocking protective autophagy.; Authors - Yu Dong
mouse	WB	Sci Rep; TRPM2 promotes autophagic degradation in vascular smooth muscle cells.; Authors - Qiannan Zhao
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human	WB	Mol Immunol; Systematic deletion of adenosine receptors reveals novel roles in inflammation and pyroptosis in THP-1 macrophages.; Authors - Brandon J Eudy
rat	WB,IF	Cell Death Dis; PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming.; Authors - Wen-Qi Ma