ATP5L Monoclonal antibody, PBS Only (Capture)

ATP5L Monoclonal Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D10F9

Cat no : 60434-1-PBS

Synonyms

ATPase subunit g, ATP5MG, ATP5JG, ATP synthase membrane subunit g, 2D10F9

---

Validation Data Gallery

• 

  IF Staining of HepG2 using 60434-1-Ig (same clone as 60434-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5L antibody (60434-1-Ig, Clone: 2D10F9 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60434-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50581-1

  Cytometric bead array standard curve of MP50581-1, ATP5L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60434-1-PBS. Detection antibody: 60434-2-PBS. Standard:Ag9287. Range: 0.391-100 ng/mL.

• 

  Cytometric bead array standard curve of MP50581-2

  Cytometric bead array standard curve of MP50581-2, ATP5L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60434-1-PBS. Detection antibody: 60434-3-PBS. Standard:Ag9287. Range: 0.391-100 ng/mL.

---

Product Information

60434-1-PBS targets ATP5L as part of a matched antibody pair:

MP50581-1: 60434-1-PBS capture and 60434-2-PBS detection (validated in Cytometric bead array)

MP50581-2: 60434-1-PBS capture and 60434-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP5L fusion protein Ag9287
• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G
• Calculated Molecular Weight: 11 kDa
• GenBank Accession Number: BC015128
• Gene Symbol: ATP5L
• Gene ID (NCBI): 10632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G Magarose purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5L gene encodes ATP synthase subunit g of the Fo complex.