ATP5H Polyclonal antibody

ATP5H Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17589-1-AP


ATP5H, ATP5JD, ATPase subunit d, ATPQ

Tested Applications

Positive WB detected inJurkat cells, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17589-1-AP targets ATP5H in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP5H fusion protein Ag11429
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
Calculated Molecular Weight 137 aa, 16 kDa
Observed Molecular Weight 19-22 kDa
GenBank Accession NumberBC032245
Gene Symbol ATP5H
Gene ID (NCBI) 10476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.


Product Specific Protocols
WB protocol for ATP5H antibody 17589-1-APDownload protocol
IHC protocol for ATP5H antibody 17589-1-APDownload protocol
IF protocol for ATP5H antibody 17589-1-APDownload protocol
IP protocol for ATP5H antibody 17589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy.

Authors - Xia Feng


AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages.

Authors - Liangjun Xia

Brain Pathol

Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus.

Authors - Margalida A Frau-Méndez

Oncol Lett

Dihydroartemisinin inhibits liver cancer cell migration and invasion by reducing ATP synthase production through CaMKK2/NCLX

Authors - Jiang Chang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


SITING (Verified Customer) (05-25-2021)


  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EMBRYO HEART
ATP5H Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:17589-1-AP)

Zee (Verified Customer) (01-28-2020)

It worked well with western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue extract