ATP5G3 Polyclonal antibody

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  IHC staining of human ovary tumor using 21662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21662-1-AP (ATP5G3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 21662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21662-1-AP (ATP5G3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP5G3 Polyclonal Antibody for IHC, ELISA

Cat No. 21662-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

ATP synthase proteolipid P3, ATP5G3, ATPase protein 9, ATPase subunit c, P3

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Tested Applications

• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21662-1-AP targets ATP5G3 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP5G3 fusion protein Ag16406
• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9)
• Calculated Molecular Weight: 142 aa, 15 kDa
• GenBank Accession Number: BC106881
• Gene Symbol: ATP5G3
• Gene ID (NCBI): 518
• RRID: AB_3085665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48201
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP5G3, also known as ATP5MC3, belongs to the ATPase C chain family. ATP5G3 encodes subunit 9 (ATPase subunit c), which is a subunit of the multi-subunit enzyme that catalyzes ATP synthesis during oxidative phosphorylation in mitochondria. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The subunit c is a component of the proton channel proteins in the F0 portion of the enzyme.