ATP5D Monoclonal antibody, PBS Only

ATP5D Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12C7

Cat no : 66673-1-PBS

Synonyms

ATP5D, F ATPase delta subunit

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Validation Data Gallery

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  WB analysis using 66673-1-Ig (same clone as 66673-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66673-1-Ig (ATP5D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66673-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66673-1-Ig (same clone as 66673-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66673-1-Ig (ATP5D antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66673-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66673-1-Ig (same clone as 66673-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66673-1-Ig (ATP5D antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66673-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66673-1-PBS targets ATP5D in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP5D fusion protein Ag7016
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC002389
• Gene Symbol: ATP5D
• Gene ID (NCBI): 513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.