ATP5C1 Polyclonal antibody

ATP5C1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10910-1-AP

Synonyms

F-ATPase gamma subunit, ATP5F1C, ATP5CL1, ATP5C, ATP synthase F1 subunit gamma

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Validation Data Gallery

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  WB analysis using 10910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10910-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 10910-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10910-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10910-1-AP (ATP5C1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L); Phalloidin stains F-actin (Red).

• 

  IF Staining of HepG2 using 10910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10910-1-AP (ATP5C1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IP: See 1 publications below

Product Information

10910-1-AP targets ATP5C1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP5C1 fusion protein Ag1343
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC000931
• Gene Symbol: ATP5C1
• Gene ID (NCBI): 509
• RRID: AB_2877740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB,IP	Autophagy; AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.; Authors - Zhenhong Ni
human	WB	J Biol Chem; An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.; Authors - Xiaofen Jin
mouse	WB	PLoS One; Warburg effect's manifestation in aggressive pheochromocytomas and paragangliomas: insights from a mouse cell model applied to human tumor tissue.; Authors - Stephanie M J Fliedner
human	WB	FEBS Lett; Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.; Authors - Makoto Fujikawa
human	WB	iScience; Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis; Authors - Yunfan He