Various lysates were subjected to SDS PAGE followed by western blot with 82288-1-RR (ATP5A1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82288-1-RR (ATP5A1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 82288-1-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 82288-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 82288-1-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 82288-1-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 82288-1-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 82288-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 82288-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 82288-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 82288-1-RR
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5A1 (82288-1-RR, Clone:4O12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5A1 (82288-1-RR, Clone:4O12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 82288-1-RR
Biolayer interferometry (BLl) kinetic assays of 82288-1-RR against Human ATP5A1 were performed. The affinity constant is 2.37 nM.
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mouse brain tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells, HeLa cells
Positive FC (Intra) detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:1100-1:4400
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
82288-1-RR targets ATP5A1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo-containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.
Various lysates were subjected to SDS PAGE followed by western blot with 82288-1-RR (ATP5A1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver using 82288-1-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 82288-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 82288-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 82288-1-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 82288-1-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 82288-1-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 82288-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 82288-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 82288-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of HeLa using 82288-1-RR
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5A1 (82288-1-RR, Clone:4O12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 82288-1-RR
Biolayer interferometry (BLl) kinetic assays of 82288-1-RR against Human ATP5A1 were performed. The affinity constant is 2.37 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ATP5A1 Recombinant antibody
Catalog Number
82288-1-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF/ICC : 1:1100-1:4400 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
