ATP4A Recombinant antibody

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  WB analysis using 83079-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83079-5-RR (ATP4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ATP4A {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83079-5-RR

Clone No.230313C10

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

ATP4A, ATP6A, Proton pump

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Tested Applications

• Positive WB detected in: rat stomach tissue, mouse stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83079-5-RR targets ATP4A in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATPase, H+/K+ exchanging, alpha polypeptide
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: NM_000704
• Gene Symbol: ATP4A
• Gene ID (NCBI): 495
• RRID: AB_3670796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20648
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. ATP4A belongs to a family of P-type cation-transporting ATPases, encodes a catalytic alpha subunit of the gastric H+, K+-ATPase, and transports H+ ions in exchange for K+ ions across the apical membrane of parietal cells.