ATP2B1 Recombinant antibody

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  WB analysis of mouse brain using 83827-3-RR

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 83827-3-RR (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse small intestine using 83827-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83827-3-RR (ATP2B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83827-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83827-3-RR against Human ATP2B1 were performed. The affinity constant is 10.3 nM.

ATP2B1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 83827-3-RR

Clone No.240848D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PMCA1kb, PMCA1, 240848D10

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Tested Applications

• Positive WB detected in: unboiled mouse brain tissue
• Positive IHC detected in: mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83827-3-RR targets ATP2B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATP2B1 fusion protein Ag32423
• Full Name: ATPase, Ca++ transporting, plasma membrane 1
• Calculated Molecular Weight: 135 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_001001323.1
• Gene Symbol: ATP2B1
• Gene ID (NCBI): 490
• RRID: AB_3671411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20020
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).