SERCA3 Polyclonal antibody

SERCA3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13619-1-AP

Synonyms

ATP2A3, Calcium pump 3, SERCA3, SR Ca(2+) ATPase 3

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Validation Data Gallery

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  WB analysis using 13619-1-AP

  Mouse heart lysates were subjected to SDS PAGE followed by western blot with 13619-1-AP (SERCA3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13619-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13619-1-AP (SERCA3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 13619-1-AP

  IP result of anti-SERCA3 (IP:13619-1-AP, 4ug; Detection:13619-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

• 

  IHC staining of human pancreas cancer using 13619-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13619-1-AP (SERCA3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13619-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13619-1-AP (SERCA3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, K-562 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13619-1-AP targets SERCA3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERCA3 fusion protein Ag4527
• Full Name: ATPase, Ca++ transporting, ubiquitous
• Calculated Molecular Weight: 1043 aa, 114 kDa
• Observed Molecular Weight: 97-110 kDa
• GenBank Accession Number: BC035729
• Gene Symbol: SERCA3
• Gene ID (NCBI): 489
• RRID: AB_2061448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The SERCA, which pumps cytosolic Ca2+ into the ER, is an imperative maintainer of ER Ca2+ homeostasis. The mammalian SERCA family is comprised of three tissue-specific members, SERCA1-3. SERCA3 is present in endothelial and epithelial cells of various tissues. SERCA3 helps regulate intracellular Ca2+, which is critical for maintaining normal INS secretion and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. Several isoforms of SERCA3 exist due to the alternative splicing.

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Astragaloside IV Attenuates Podocyte Apoptosis Mediated by Endoplasmic Reticulum Stress through Upregulating Sarco/Endoplasmic Reticulum Ca(2+)-ATPase 2 Expression in Diabetic Nephropathy.; Authors - Hengjiang Guo
human	WB	Acta Pharm Sin B; Highly expressed SERCA2 triggers tumor cell autophagy and is a druggable vulnerability in triple-negative breast cancer; Authors - Minmin Fan
mouse	IHC,WB	Nat Commun; Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics; Authors - Xi Shao
mouse	WB	Front Physiol; Proteomic analyses of urinary exosomes identify novel potential biomarkers for early diagnosis of sickle cell nephropathy, a sex-based study; Authors - Balamurugan Packialakshmi
mouse	IF	Sci China Life Sci; The single-cell transcriptomic landscape of the topological differences in mammalian auditory receptors; Authors - Xiangyu Ma