ATP1A3 (middle) Polyclonal antibody

ATP1A3 (middle) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28030-1-AP

Synonyms

ATP1A3, ATP1A3 (middle), DYT12, RDP, Sodium pump subunit alpha 3

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Validation Data Gallery

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  WB analysis of HeLa using 28030-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28030-1-AP (ATP1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28030-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28030-1-AP (ATP1A3 (middle) antibody) at dilution of 1:14000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 28030-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28030-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 28030-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28030-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28030-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 28030-1-AP (ATP1A3 (middle) antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, SKOV-3 cells, U-87 MG cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

28030-1-AP targets ATP1A3 (middle) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP1A3 (middle) fusion protein Ag27705
• Full Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 113 kDa
• GenBank Accession Number: BC015566
• Gene Symbol: ATP1A3
• Gene ID (NCBI): 478
• RRID: AB_2881041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na⁺(In) + K⁺(Out) = ADP + phosphate + Na⁺(Out) + K⁺(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na⁺/K⁺ homeostasis. This antibody is generated agaist the middle region (378-580aa) of ATP1A3 and detects 113 kDa protein in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)

Publications

Species	Application	Title
human	WB, IF	Sci Adv; De novo ATP1A3 variants cause polymicrogyria.; Authors - Satoko Miyatake
human	WB	Nat Commun; Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.; Authors - Yi-Ing Chen