ATP1A3 (C-terminal) Polyclonal antibody

ATP1A3 (C-terminal) Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10868-1-AP

Synonyms

ATP1A3 (middle), ATP1A3, EC:7.2.2.13, DYT12, ATP1A 3

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Validation Data Gallery

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  WB analysis using 10868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 10868-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 10868-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 10868-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C2C12 cell, mouse brain tissue, C2C12 cells
• Positive IHC detected in: human prostate cancer tissue, mouse brain tissue, mouse skeletal muscle tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

10868-1-AP targets ATP1A3 (C-terminal) in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP1A3 (C-terminal) fusion protein Ag1313
• Full Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 100-113 kDa
• GenBank Accession Number: BC015566
• Gene Symbol: ATP1A3
• Gene ID (NCBI): 478
• RRID: AB_2060962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na⁺(In) + K⁺(Out) = ADP + phosphate + Na⁺(Out) + K⁺(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na⁺/K⁺ homeostasis. This antibody is generated against the C-terminal region (665-1013aa) of ATP1A3 and detects the band around 100-113 kDa in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)

Publications

Species	Application	Title
human	WB	Front Oncol; Elevated Sodium Pump α3 Subunit Expression Promotes Colorectal Liver Metastasis via the p53-PTEN/IGFBP3-AKT-mTOR Axis.; Authors - Di Wu
human	IHC	Cancer Cell Int; Comprehensive analysis of the expression of sodium/potassium-ATPase α subunits and prognosis of ovarian serous cystadenocarcinoma.; Authors - Wei Huang
human	WB,IHC	Life Sci; Identification of a robust scoring system based on metabolic genes followed by in-depth validation of ATP1A3 in glioma; Authors - Qiankun Ji