ATM Monoclonal antibody

ATM Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

3E6F4

Cat no : 67586-1-Ig

Synonyms

A T mutated, AT1, ATA, ataxia telangiectasia mutated, ATC, ATD, ATDC, ATE, ATM, DKFZp781A0353, Serine protein kinase ATM, TEL1, TELO1

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Validation Data Gallery

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  WB analysis using 67586-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67586-1-Ig (ATM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67586-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67586-1-Ig (ATM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67586-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67586-1-Ig (ATM antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

67586-1-Ig targets ATM in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATM fusion protein Ag30001
• Full Name: ataxia telangiectasia mutated
• Observed Molecular Weight: 310-350 kDa
• GenBank Accession Number: BC137169
• Gene Symbol: ATM
• Gene ID (NCBI): 472
• RRID: AB_2882795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

Publications

Species	Application	Title
human	WB	Food Chem Toxicol; DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells; Authors - Silu Hou
rat	IF,WB	CNS Neurosci Ther; Multiple sevoflurane exposures during mid-trimester induce neurotoxicity in the developing brain initiated by 15LO2-Mediated ferroptosis; Authors - Qian Jiang
human	WB	J Mol Med (Berl); High expression of BCAT1 sensitizes AML cells to PARP inhibitor by suppressing DNA damage response; Authors - Jiajia Pan