ATL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16688-1-AP

Synonyms

ARL-6-interacting protein 2, ARL3IP2, ARL 6 interacting protein 2, Aip-2, Aip 2

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Validation Data Gallery

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  WB analysis using 16688-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16688-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16688-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16688-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 16688-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16688-1-AP (ATL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 16688-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16688-1-AP (ATL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 16688-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16688-1-AP (ATL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 16688-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16688-1-AP (ATL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, HeLa cells, Jurkat cells, HepG2 cells, SH-SY5Y cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Product Information

16688-1-AP targets ATL2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATL2 fusion protein Ag10030
• Full Name: atlastin GTPase 2
• Calculated Molecular Weight: 412aa,47 kDa; 583aa,66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC053508
• Gene Symbol: ATL2
• Gene ID (NCBI): 64225
• RRID: AB_1850898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATL2, also known as Atlastin GTPase 2, is involved in various cellular processes, including the organization of the Golgi apparatus, the endoplasmic reticulum tubular network membrane organization, and protein homooligomerization. It is located in the endoplasmic reticulum tubular network membrane and is an integral component of the membrane. The ATL2 gene has multiple transcripts and isoforms, with 18 transcripts (splice variants), 297 orthologues, and 10 paralogues. ATL2 also plays a significant role in the immune response, particularly in plants. In Arabidopsis thaliana, the ATL2 gene is involved in the response to fungal pathogen infection. The expression of ATL2 is low under normal growth conditions but is rapidly and significantly induced by exogenous chitin.also plays

Publications

Species	Application	Title
human	IF	J Cell Biol; Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.; Authors - Nan Liu
human,mouse,monkey	WB	J Cell Biol; Human atlastin-3 is a constitutive ER membrane fusion catalyst; Authors - Samantha Bryce
mouse	WB	Nature; Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy; Authors - Hector Foronda
human	WB	Nat Struct Mol Biol; Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis; Authors - Wei Zhang
human	WB,IF	J Biochem; Lunapark ubiquitinates atlastin-2 for the tubular network formation of the endoplasmic reticulum.; Authors - Toshiaki Sakisaka
mouse	WB	NPJ Parkinsons Dis; Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities; Authors - Jia Yu