ATGL Recombinant antibody

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  WB analysis using 84736-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84736-1-RR (ATGL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ATGL Recombinant Antibody for WB, ELISA

Cat No. 84736-1-RR

Clone No.241919C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Adipose triglyceride lipase, PNPLA2, 241919C6, EC:3.1.1.4, EC:3.1.1.3

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, 3T3-L1 cells, NIH/3T3 cells, mouse heart tissue, mouse testis tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84736-1-RR targets ATGL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: patatin-like phospholipase domain containing 2
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_020376
• Gene Symbol: ATGL
• Gene ID (NCBI): 57104
• RRID: AB_3672120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96AD5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.