ATG9A Polyclonal antibody

ATG9A Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 26276-1-AP


APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26276-1-AP targets ATG9A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG9A fusion protein Ag24212
Full Name ATG9 autophagy related 9 homolog A (S. cerevisiae)
Calculated Molecular Weight 837 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC065534
Gene Symbol ATG9A
Gene ID (NCBI) 79065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)


Product Specific Protocols
WB protocol for ATG9A antibody 26276-1-APDownload protocol
IHC protocol for ATG9A antibody 26276-1-APDownload protocol
IF protocol for ATG9A antibody 26276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cancer

PMID: 32727463

Authors - Cefan Zhou

Acta Pharm Sin B

MicroRNA-34c-5p provokes isoprenaline-induced cardiac hypertrophy by modulating autophagy via targeting ATG4B.

Authors - Yuhong Zhang

Front Immunol

MicroRNA-34a Inhibition Alleviates Lung Injury in Cecal Ligation and Puncture Induced Septic Mice.

Authors - Song Chen

DNA Cell Biol

Long Noncoding RNA KIF9-AS1 Regulates Transforming Growth Factor-β and Autophagy Signaling to Enhance Renal Cell Carcinoma Chemoresistance via microRNA-497-5p.

Authors - Yichen Jin

Mol Neurobiol

RAB7A GTPase Is Involved in Mitophagosome Formation and Autophagosome-Lysosome Fusion in N2a Cells Treated with the Prion Protein Fragment 106-126

Authors - Zhiping Li

Cell Biol Int

TFAM knockdown undermines SQSTM1 mRNA stability but retards autophagy flux and inhibits tumor cells proliferation under starvation conditions

Authors - Xu Jiang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells and mice tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon