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GABARAPL1 Recombinant antibody, PBS Only

GABARAPL1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240533F5

Cat no : 83817-1-PBS

Synonyms

Early estrogen-regulated protein, ATG8L, ATG8, APG8L, APG8 LIKE

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GABARAPL1 Recombinant antibody (83817-1-RR)

    WB analysis using 83817-1-RR (same clone as 83817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83817-1-RR (ATG8L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83817-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GABARAPL1 Recombinant antibody (83817-1-RR)

    IF Staining of HepG2 using 83817-1-RR (same clone as 83817-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATG8L antibody (83817-1-RR, Clone: 240533F5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83817-1-PBS in a different storage buffer formulation.

Product Information

The immunogen of 83817-1-PBS is GABARAPL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GABARAPL1 fusion protein Ag1473
Full Name GABA(A) receptor-associated protein like 1
Calculated Molecular Weight 14 kDa
Observed Molecular Weight16 kDa
GenBank Accession NumberBC009309
Gene Symbol GABARAPL1
Gene ID (NCBI) 23710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine.