ATG7 Recombinant antibody

• Featured Product
• KD/KO Validated

ATG7 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2M8

Cat no : 81760-1-RR

Synonyms

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7

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Validation Data Gallery

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  WB analysis of HeLa using 81760-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 81760-1-RR (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 81760-1-RR

  WB result of ATG7 antibody (81760-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells.

• 

  IP experiment of HeLa using 81760-1-RR

  IP result of anti-ATG7 (IP:81760-1-RR, 4ug; Detection:81760-1-RR 1:2000) with HeLa cells lysate 1720 ug.

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  IF Staining of HepG2 using 81760-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG7 antibody (81760-1-RR, Clone: 2M8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  Affinity and Kinetic Characterization of 81760-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81760-1-RR against Human ATG7 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, U2OS cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81760-1-RR targets ATG7 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATG7 fusion protein Ag0142
• Full Name: ATG7 autophagy related 7 homolog (S. cerevisiae)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 68-78 kDa
• GenBank Accession Number: BC000091
• Gene Symbol: ATG7
• Gene ID (NCBI): 10533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.