ATG5 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ATG5 Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D3B8

Cat no : 66744-1-PBS

Synonyms

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

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Validation Data Gallery

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  WB analysis using 66744-1-Ig (same clone as 66744-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66744-1-Ig (same clone as 66744-1-PBS)

  WB result of ATG5 antibody (66744-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HeLa cells. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66744-1-Ig (same clone as 66744-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 66744-1-Ig (same clone as 66744-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human ATG5 (66744-1-Ig, Clone:2D3B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66744-1-PBS targets ATG5 in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATG5 fusion protein Ag16685
• Full Name: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 50-55 and 40-45 kDa
• GenBank Accession Number: BC002699
• Gene Symbol: ATG5
• Gene ID (NCBI): 9474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)