Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis using 67727-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HuH-7 using 67727-1-Ig
WB result of ATG4B antibody (67727-1-Ig; 1:800; room temperature for 1.5 hours) with wild-type and ATG4B knockout HuH-7 cells.
WB result of ATG4B antibody (67727-1-Ig; 1:800; room temperature for 1.5 hours) with wild-type and ATG4B knockout HuH-7 cells.
WB analysis of HSC-T6 using 67727-1-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 67727-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 67727-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 67727-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 67727-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATG4B antibody (67727-1-Ig, Clone: 1G5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATG4B antibody (67727-1-Ig, Clone: 1G5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
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human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.
Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis using 67727-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HuH-7 using 67727-1-Ig
WB result of ATG4B antibody (67727-1-Ig; 1:800; room temperature for 1.5 hours) with wild-type and ATG4B knockout HuH-7 cells.
WB analysis of HSC-T6 using 67727-1-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human kidney using 67727-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 67727-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 67727-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 67727-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATG4B antibody (67727-1-Ig, Clone: 1G5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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