ATG4A Polyclonal antibody

ATG4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27467-1-AP

Synonyms

APG4A, ATG4A, AUTL2, Autophagin 2, Cysteine protease ATG4A, hAPG4A

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Validation Data Gallery

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  WB analysis of L02 using 27467-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 27467-1-AP (ATG4A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MKN-45 using 27467-1-AP

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 27467-1-AP (ATG4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 27467-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27467-1-AP (ATG4A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27467-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27467-1-AP (ATG4A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: L02 cells, MKN-45 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1500
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27467-1-AP targets ATG4A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG4A fusion protein Ag26497
• Full Name: ATG4 autophagy related 4 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC061696
• Gene Symbol: ATG4A
• Gene ID (NCBI): 115201
• RRID: AB_2880879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG4, a member of the cysteine protease family, plays an important role in autophagy induction. ATG4 enzymes cleave the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine that is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes. ATG4A, which may be a target for cancer prevention and intervention, has been reported to be related to breast tumorigenesis, lung cancer, ovarian cancer, cervical cancer and gastric cancer. (PMID: 27276686, 29435029, 28636635)

Publications

Species	Application	Title
human	WB	Cell Death Dis; The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis.; Authors - Zhenhong Ni