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  • KD/KO Validated

ATG2A Polyclonal antibody

ATG2A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Peptide array, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 23226-1-AP

Synonyms

ATG2A, KIAA0404



Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23226-1-AP targets ATG2A in WB, Peptide array, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG2A fusion protein Ag19709
Full Name ATG2 autophagy related 2 homolog A (S. cerevisiae)
Calculated Molecular Weight 1938 aa, 213 kDa
Observed Molecular Weight 215 kDa
GenBank Accession NumberBC110650
Gene Symbol ATG2A
Gene ID (NCBI) 23130
RRIDAB_2879235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ATG2A antibody 23226-1-APDownload protocol
IHC protocol for ATG2A antibody 23226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
humanWB

Nat Cell Biol

ATG9A protects the plasma membrane from programmed and incidental permeabilization.

Authors - Aurore Claude-Taupin
  • KO Validated
humanWB,CoIP,Peptide array

Mol Cell

ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation

Authors - Alexander R van Vliet
humanIF

Nat Commun

The autophagy protein ATG9A enables lipid mobilization from lipid droplets.

Authors - Elodie Mailler
  • KO Validated
humanWB,IF

EMBO Rep

A conserved ATG2-GABARAP family interaction is critical for phagophore formation.

Authors - Mihaela Bozic
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun