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  • KD/KO Validated

ATG2A Polyclonal antibody

ATG2A Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG




WB, IF, IHC, CoIP, ELISA, Peptide array



Cat no : 23226-1-AP



Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23226-1-AP targets ATG2A in WB, IF, IHC, CoIP, ELISA, Peptide array applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG2A fusion protein Ag19709
Full Name ATG2 autophagy related 2 homolog A (S. cerevisiae)
Calculated Molecular Weight 1938 aa, 213 kDa
Observed Molecular Weight 215 kDa
GenBank Accession NumberBC110650
Gene Symbol ATG2A
Gene ID (NCBI) 23130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Autophagy-related protein 2 homolog A (ATG2A) belongs to the ATG2 family. ATG2A is a lipid transfer protein involved in autophagosome assembly (PMID:31271352). ATG2A tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. ATG2A binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PMID: 30952800, PMID: 31271352). ATG2A also regulates lipid droplet morphology and distribution within the cell (PMID: 28561066). The observed MW of ATG2A is 215 kDa.


Product Specific Protocols
WB protocol for ATG2A antibody 23226-1-APDownload protocol
IHC protocol for ATG2A antibody 23226-1-APDownload protocol
IF protocol for ATG2A antibody 23226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan

Nat Cell Biol

ATG9A protects the plasma membrane from programmed and incidental permeabilization.

Authors - Aurore Claude-Taupin
  • KO Validated
humanWB,CoIP,Peptide array

Mol Cell

ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation

Authors - Alexander R van Vliet

Nat Commun

The autophagy protein ATG9A enables lipid mobilization from lipid droplets.

Authors - Elodie Mailler
  • KO Validated


A conserved ATG2-GABARAP family interaction is critical for phagophore formation.

Authors - Mihaela Bozic


The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun