• Featured Product
  • KD/KO Validated

ATG2A Polyclonal antibody

ATG2A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Peptide array, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 23226-1-AP

Synonyms

ATG2A, KIAA0404



Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

23226-1-AP targets ATG2A in WB, Peptide array, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG2A fusion protein Ag19709
Full Name ATG2 autophagy related 2 homolog A (S. cerevisiae)
Calculated molecular weight 1938 aa, 213 kDa
Observed molecular weight 215 kDa
GenBank accession numberBC110650
Gene symbol ATG2A
Gene ID (NCBI) 23130
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ATG2A antibody 23226-1-APDownload protocol
IHC protocol for ATG2A antibody 23226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
humanWB

Nat Cell Biol

ATG9A protects the plasma membrane from programmed and incidental permeabilization.

Authors - Aurore Claude-Taupin
  • KO Validated
humanWB,CoIP,Peptide array

Mol Cell

ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation

Authors - Alexander R van Vliet
humanIF

Nat Commun

The autophagy protein ATG9A enables lipid mobilization from lipid droplets.

Authors - Elodie Mailler
  • KO Validated
humanWB,IF

EMBO Rep

A conserved ATG2-GABARAP family interaction is critical for phagophore formation.

Authors - Mihaela Bozic
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun