ATG12 Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01715-3

  Sandwich ELISA standard curve of MP01715-3, Human ATG12 Recombinant Matched Antibody Pair - PBS only. 84935-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1791. 84935-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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  Sample test of MP01715-3

  The mean ATG12 concentration was determined to be 5,245.1 pg/mL in COLO 320 cell extract based on a 2.0 mg/mL extract load, 12,068.3 pg/mL in HeLa cell extract based on a 1.3 mg/mL extract load and 26,207.3 pg/mL in PC-3 cell extract based on a 2.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP01715-2

  Cytometric bead array standard curve of MP01715-2, ATG12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84935-4-PBS. Detection antibody: 84935-2-PBS. Standard: Ag1791. Range: 0.469-60 ng/mL.

ATG12 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84935-2-PBS

Clone No.242483D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Autophagy-related protein 12, APG12-like, APG12L, APG12 like, APG12

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Product Information

84935-2-PBS targets ATG12 as part of a matched antibody pair:

MP01715-2: 84935-4-PBS capture and 84935-2-PBS detection (validated in Cytometric bead array)

MP01715-3: 84935-1-PBS capture and 84935-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATG12 fusion protein Ag1791
• Full Name: ATG12 autophagy related 12 homolog (S. cerevisiae)
• Calculated Molecular Weight: 15 kDa
• GenBank Accession Number: BC012266
• Gene Symbol: ATG12
• Gene ID (NCBI): 9140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O94817
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.