ATG12 Polyclonal antibody

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  WB analysis using 30505-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30505-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 30505-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ATG12 antibody (30505-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ATG12 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30505-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

APG12, APG12 like, APG12L, APG12-like, Autophagy-related protein 12

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, NIH/3T3 cells, PC-3 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30505-1-AP targets ATG12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG12 fusion protein Ag32268
• Full Name: ATG12 autophagy related 12 homolog (S. cerevisiae)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 20 kDa, 50-60 kDa
• GenBank Accession Number: BC012266
• Gene Symbol: ATG12
• Gene ID (NCBI): 9140
• RRID: AB_3086340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94817
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.