ATG101 Polyclonal antibody

ATG101 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26562-1-AP

Synonyms

ATG101, Autophagy related protein 101, C12orf44

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Validation Data Gallery

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  WB analysis of mouse brain using 26562-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26562-1-AP (ATG101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 26562-1-AP

  IP result of anti-ATG101 (IP:26562-1-AP, 4ug; Detection:26562-1-AP 1:300) with HeLa cells lysate 2000ug.

• 

  IHC staining of human liver cancer using 26562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26562-1-AP (ATG101 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26562-1-AP (ATG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26562-1-AP (ATG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26562-1-AP (ATG101 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:800
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 26562-1-AP is ATG101 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG101 fusion protein Ag22932
• Full Name: chromosome 12 open reading frame 44
• Calculated Molecular Weight: 218 aa, 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC005151
• Gene Symbol: ATG101
• Gene ID (NCBI): 60673
• RRID: AB_2880554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Biol Int; TFAM knockdown undermines SQSTM1 mRNA stability but retards autophagy flux and inhibits tumor cells proliferation under starvation conditions; Authors - Xu Jiang