ATF7 Polyclonal antibody

ATF7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29770-1-AP

Synonyms

ATF7, ATFA, Transcription factor ATF A

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Validation Data Gallery

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  WB analysis using 29770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29770-1-AP (ATF7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 29770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 29770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 29770-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 29770-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 29770-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 29770-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 29770-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 29770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human lung tissue, mouse lung tissue, human urothelial carcinoma tissue, mouse heart tissue, rat heart tissue, rat lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29770-1-AP targets ATF7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATF7 fusion protein Ag30882
• Full Name: activating transcription factor 7
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 52-60 kDa
• GenBank Accession Number: NM_001366555
• Gene Symbol: ATF7
• Gene ID (NCBI): 11016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.