ATAD1 Polyclonal antibody

• 

  WB analysis using 16882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16882-1-AP (ATAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16882-1-AP

  IP result of anti-ATAD1 (IP:16882-1-AP, 4ug; Detection:16882-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of human colon cancer using 16882-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16882-1-AP (ATAD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATAD1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16882-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

hATAD1, FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1

Formulation:  PBS and Azide

PBS and Azide

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Add to Basket

Please visit your regions distributor:

---

Proteintech Guarantee

---

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16882-1-AP targets ATAD1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATAD1 fusion protein Ag10343
• Full Name: ATPase family, AAA domain containing 1
• Calculated Molecular Weight: 361 aa, 41 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC010868
• Gene Symbol: ATAD1
• Gene ID (NCBI): 84896
• RRID: AB_2878327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NBU5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.