ASPN Polyclonal antibody

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  WB analysis of HepG2 using 27602-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27602-1-AP (ASPN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27602-1-AP (ASPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 27602-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27602-1-AP (ASPN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ASPN Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27602-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

SLRR1C, PLAP-1, PLAP1, PLAP 1, Periodontal ligament-associated protein 1

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Tested Applications

• Positive WB detected in: HepG2 cells, SW480 cells, HeLa cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27602-1-AP targets ASPN in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASPN fusion protein Ag26380
• Full Name: asporin
• Calculated Molecular Weight: 384 aa, 44 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC063114
• Gene Symbol: ASPN
• Gene ID (NCBI): 54829
• RRID: AB_3085974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXN1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASPN, also known as periodontal ligament-associated protein1 (PLAP1), is an extracellular matrix (ECM) protein. The calculated molecular weight of ASPN is 43 kDa. It belongs to the small leucine-rich proteoglycans (SLRPs) family and contains a unique aspartate-rich N terminus that distinguishes it from other SLRPs family members. It was firstly identified in human cartilage and it was associated with the pathogenesis of bone and joint diseases, including osteoarthritis, intervertebral disc degeneration and periodontal ligament mineralization (PMID:11152692; 26089687).