ASPH Polyclonal antibody

• Featured Product
• KD/KO Validated

ASPH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14116-1-AP

Synonyms

AAH, ASP beta hydroxylase, aspartate beta hydroxylase, ASPH, BAH, CASQ2BP1, HAAH, JCTN, junctin

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Validation Data Gallery

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  WB analysis of A549 using 14116-1-AP

  WB result of ASPH antibody (14116-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASPH transfected A549 cells.

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  WB analysis of mouse brain using 14116-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14116-1-AP (ASPH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14116-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14116-1-AP (ASPH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14116-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14116-1-AP (ASPH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14116-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14116-1-AP (ASPH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14116-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14116-1-AP (ASPH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells, human brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

14116-1-AP targets ASPH in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASPH fusion protein Ag5265
• Full Name: aspartate beta-hydroxylase
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC066929
• Gene Symbol: ASPH
• Gene ID (NCBI): 444
• RRID: AB_2060148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aspartate-β-hydroxylase (ASPH; termed AAH in older literature) has been implicated in the cross-talk among all of these signaling pathways. Correspondingly, ASPH is expressed at high levels in many malignant neoplasms of different histogeneses. It has the 86-141 kDa bands (native and phosphorylated forms), cleavage products of 35-56 and 22-26 kDa.(PMID:27981247/PMID: 10956665)

Publications

Species	Application	Title
human	WB	EBioMedicine; Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway.; Authors - Qifei Zou ; KD Validated
human	WB	J Immunol Res; Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma.; Authors - Yujiao Zhou
human,rat	WB,IHC	Heliyon; Labyrinthin: A distinct pan-adenocarcinoma diagnostic and immunotherapeutic tumor specific antigen.; Authors - Michael Babich
human	WB	Biochem Pharmacol; Inhibition of circular RNA ASPH reduces the proliferation and promotes the apoptosis of hepatic stellate cells in hepatic fibrosis; Authors - Hongwu Meng