ASPA Polyclonal antibody

• Featured Product
• KD/KO Validated

ASPA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13244-1-AP

Synonyms

ACY 2, ACY2, Aminoacylase 2, ASP, ASPA, Aspartoacylase

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Validation Data Gallery

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  WB analysis of mouse brain using 13244-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13244-1-AP (ASPA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 13244-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13244-1-AP (ASPA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13244-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13244-1-AP (ASPA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13244-1-AP targets ASPA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASPA fusion protein Ag4029
• Full Name: aspartoacylase (Canavan disease)
• Calculated Molecular Weight: 313 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC029128
• Gene Symbol: ASPA
• Gene ID (NCBI): 443
• RRID: AB_2274358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASPA(Aspartoacylase) also called aminoacylase-2, is an enzyme that hydrolyzes N-acetyl-L-aspartic acid (NAA) to aspartate and acetate.Human ASPA encodes a deduced 313-amino acid protein with a molecular mass of 36 KDa. It shares 92% sequence identity with the bovine protein and contains 1 potential N-glycosylation site and 5 phosphorylation sites.Defects in ASPA are the cause of Canavan disease (CAND).This protein can exsit as a homodimer(18293939).

Publications

Species	Application	Title
mouse,human	WB,IHC,IF	Mil Med Res; Aspartoacylase suppresses prostate cancer progression by blocking LYN activation; Authors - Hong Weng
human,mouse	WB	Elife; Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.; Authors - Vasiliki-Ilya Gargareta
mouse	WB	Glia; Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome; Authors - Sophie B Siems
mouse	WB	J Neurosci; Suppressing N-Acetyl-l-Aspartate Synthesis Prevents Loss of Neurons in a Murine Model of Canavan Leukodystrophy.; Authors - Jiho Sohn ; KO Validated
rat	WB	Front Pharmacol; Increasing Aspartoacylase in the Central Amygdala: The Common Mechanism of Gastroprotective Effects of Monoamine-Based Antidepressants Against Stress.; Authors - Kaiyun Yao