ASNA1 Monoclonal antibody, PBS Only (Capture)

ASNA1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, chicken

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7C11

Cat no : 66346-1-PBS

Synonyms

TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase

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Validation Data Gallery

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  WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 66346-1-Ig (same clone as 66346-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66346-1-Ig (same clone as 66346-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66346-1-Ig (same clone as 66346-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66346-1-Ig (same clone as 66346-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66346-1-Ig (same clone as 66346-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66346-1-Ig (ASNA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66346-1-Ig (same clone as 66346-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66346-1-Ig (ASNA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66346-1-Ig (same clone as 66346-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ASNA1 antibody (66346-1-Ig, Clone: 1B7C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66346-1-Ig (same clone as 66346-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ASNA1 antibody (66346-1-Ig, Clone: 1B7C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50801-1

  Cytometric bead array standard curve of MP50801-1, ASNA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66346-1-PBS. Detection antibody: 66346-2-PBS. Standard:Ag7936. Range: 0.391-100 ng/mL.

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  Cytometric bead array standard curve of MP50801-2

  Cytometric bead array standard curve of MP50801-2, ASNA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66346-1-PBS. Detection antibody: 66346-3-PBS. Standard:null. Range: null.

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Product Information

66346-1-PBS targets ASNA1 as part of a matched antibody pair:

MP50801-1: 66346-1-PBS capture and 66346-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASNA1 fusion protein Ag7936
• Full Name: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• Calculated Molecular Weight: 38.7 kDa
• Observed Molecular Weight: 37-41 kDa
• GenBank Accession Number: BC002651
• Gene Symbol: ASNA1
• Gene ID (NCBI): 439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.