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ASL Monoclonal antibody, PBS Only

ASL Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G9H3

Cat no : 67692-1-PBS

Synonyms

argininosuccinate lyase, Arginosuccinase, ASAL, ASL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASL Monoclonal antibody (67692-1-Ig)

    WB analysis using 67692-1-Ig (same clone as 67692-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67692-1-Ig (ASL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using ASL Monoclonal antibody (67692-1-Ig)

    WB analysis of LNCaP using 67692-1-Ig (same clone as 67692-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67692-1-Ig (ASL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using ASL Monoclonal antibody (67692-1-Ig)

    WB analysis of K-562 using 67692-1-Ig (same clone as 67692-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67692-1-Ig (ASL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67692-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67692-1-PBS targets ASL in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ASL fusion protein Ag30517
Full Name argininosuccinate lyase
Calculated Molecular Weight 55 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC008195
Gene Symbol ASL
Gene ID (NCBI) 435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)