ASK1 Monoclonal antibody

ASK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

1C3A10

Cat no : 67072-1-Ig

Synonyms

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5

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Validation Data Gallery

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  WB analysis of HSC-T6 using 67072-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67072-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67072-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67072-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67072-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67072-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of RAW 264.7 using 67072-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using ASK1 antibody (67072-1-Ig, Clone: 1C3A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HSC-T6 cells, HEK-293 cells, Jurkat cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
• Positive IHC detected in: human pancreas cancer tissue, mouse heart tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 3 publications below

Product Information

67072-1-Ig targets ASK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASK1 fusion protein Ag5818
• Full Name: mitogen-activated protein kinase kinase kinase 5
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC054503
• Gene Symbol: ASK1
• Gene ID (NCBI): 4217
• RRID: AB_2882381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer; Authors - Yurou Chen
human	WB	Oncol Rep; ABT‑737 increases cisplatin sensitivity through the ROS‑ASK1‑JNK MAPK signaling axis in human ovarian cancer cisplatin‑resistant A2780/DDP cells; Authors - Xiaoning Li
human,rat	WB	Aging (Albany NY); Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF; Authors - Xiao-Lin Jiang
rat	WB	Front Endocrinol (Lausanne); Cangfudaotan decoction inhibits mitochondria-dependent apoptosis of granulosa cells in rats with polycystic ovarian syndrome; Authors - Xiao-Lin Jiang
human	WB	Cells; Orphan Nuclear Receptor Nur77 Mediates the Lethal Endoplasmic Reticulum Stress and Therapeutic Efficacy of Cryptomeridiol in Hepatocellular Carcinoma; Authors - Xudan Li
rat	IF	Mol Med Rep; ASK1 inhibitor NQDI‑1 decreases oxidative stress and neuroapoptosis via the ASK1/p38 and JNK signaling pathway in early brain injury after subarachnoid hemorrhage in rats; Authors - Jiajia Duan