ASGR2 Monoclonal antibody

ASGR2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A5C12

Cat no : 66831-1-Ig

Synonyms

ASGP R, ASGP R 2, ASGPR 2, ASGR2, asialoglycoprotein receptor 2, CLEC4H2, Hepatic lectin H2, HL 2, Hs.1259, L H2

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Validation Data Gallery

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  WB analysis of SMMC-7721 using 66831-1-Ig

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 66831-1-Ig (ASGR2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66831-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66831-1-Ig (ASGR2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SMMC-7721 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66831-1-Ig targets ASGR2 in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASGR2 fusion protein Ag2037
• Full Name: asialoglycoprotein receptor 2
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC017251
• Gene Symbol: ASGR2
• Gene ID (NCBI): 433
• RRID: AB_2882174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.