ASGR1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ASGR1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G5F5

Cat no : 66692-1-PBS

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

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Validation Data Gallery

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  WB analysis of HepG2 using 66692-1-Ig (same clone as 66692-1-PBS)

  WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected HepG2 cells. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

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  WB analysis using 66692-1-Ig (same clone as 66692-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66692-1-Ig (same clone as 66692-1-PBS)

  WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected L02 cells. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66692-1-Ig (same clone as 66692-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66692-1-Ig (same clone as 66692-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse hepatocytes using 66692-1-Ig (same clone as 66692-1-PBS)

  mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66692-1-Ig (same clone as 66692-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66692-1-Ig (same clone as 66692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66692-1-Ig (same clone as 66692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66692-1-Ig (same clone as 66692-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using ASGR1 antibody (66692-1-Ig, Clone: 1G5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

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Product Information

66692-1-PBS targets ASGR1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASGR1 fusion protein Ag2310
• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 291 aa, 33 kDa
• Observed Molecular Weight: 42-46 kDa
• GenBank Accession Number: BC032130
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.