ASGR1 Polyclonal antibody

ASGR1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 31338-1-AP

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

---

Validation Data Gallery

• 

  WB analysis using 31338-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31338-1-AP (ASGR1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat liver using 31338-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 31338-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 31338-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 31338-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat liver using 31338-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (31338-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat liver using 31338-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (31338-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: rat liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31338-1-AP targets ASGR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: NM_001671.4
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.