ASF1A Polyclonal antibody

ASF1A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10784-1-AP

Synonyms

ASF1A, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10784-1-AP targets ASF1A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASF1A fusion protein Ag1233
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC010878
Gene Symbol ASF1A
Gene ID (NCBI) 25842
RRIDAB_2243015
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

Protocols

Product Specific Protocols
WB protocol for ASF1A antibody 10784-1-APDownload protocol
IHC protocol for ASF1A antibody 10784-1-APDownload protocol
IP protocol for ASF1A antibody 10784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP

J Virol

Varicella-zoster virus immediate-early 63 protein interacts with human antisilencing function 1 protein and alters its ability to bind histones h3.1 and h3.3.

Authors - Ambagala Aruna P AP
mouseWB,IF

Epigenetics Chromatin

Distinct role of histone chaperone Asf1a and Asf1b during fertilization and pre-implantation embryonic development in mice.

Authors - Xuemei Wang
humanIF

PLoS One

HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

Authors - Jiang Wei-Qin WQ
humanIHC,WB

Sci Rep

Identification of ASF1A and HJURP by global H3-H4 histone chaperone analysis as a prognostic two-gene model in hepatocellular carcinoma

Authors - Yongkang Liu