ASCL2 Polyclonal antibody

ASCL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21368-1-AP

Synonyms

Class A basic helix-loop-helix protein 45, bHLHa45, ASH-2, ASH2, Achaete-scute homolog 2

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Validation Data Gallery

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  WB analysis of Caco-2 using 21368-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 21368-1-AP (ASCL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MKN-45 using 21368-1-AP

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 21368-1-AP (ASCL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 21368-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21368-1-AP (ASCL2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MKN-45 cells, Caco-2 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

21368-1-AP targets ASCL2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASCL2 fusion protein Ag15839
• Full Name: achaete-scute complex homolog 2 (Drosophila)
• Calculated Molecular Weight: 193 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC057801
• Gene Symbol: ASCL2
• Gene ID (NCBI): 430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Achaete-scute complex homolog 2 (Drosophila), also known as ASCL2, is an imprinted human gene. This gene is involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system, particularly important during implantation of the developing embryo, specifically in placental development and neuronal precursor determination. ASCL2 has been reported involved in tumor progression, being upregulated in colorectal tumors, and involvement in metastasis could be attributed to ASCL2-promoted cellular self-renewal as opposed to cellular differentiation (PMID: 30096149 16568095).

Publications

Species	Application	Title
human	WB	Gene; tRNA-derived RNA fragment, tRF-18-8R6546D2, promotes pancreatic adenocarcinoma progression by directly targeting ASCL2; Authors - Sihua Lan