Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IP | See 2 publications below |
Product Information
17627-1-AP targets ASCC3 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ASCC3 fusion protein Ag11848 Predict reactive species |
| Full Name | activating signal cointegrator 1 complex subunit 3 |
| Calculated Molecular Weight | 111 aa, 13 kDa, 251 kDa |
| Observed Molecular Weight | 251 kDa |
| GenBank Accession Number | BC050681 |
| Gene Symbol | ASCC3 |
| Gene ID (NCBI) | 10973 |
| RRID | AB_2059474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N3C0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ASCC3 (Activating Signal Cointegrator 1 Complex Subunit 3) is a multifunctional protein involved in various cellular processes, including transcriptional regulation, DNA repair, and ribosome quality control. It is a member of the ASCC (Activating Signal Cointegrator 1 Complex) family, which functions as a bridge between co-repressors and co-activators in transcriptional regulation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASCC3 antibody 17627-1-AP | Download protocol |
| IP protocol for ASCC3 antibody 17627-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex | ||
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Mol Cell Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress. | ||
Sci Rep Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells. | ||
Cell Metab Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tom (Verified Customer) (10-24-2023) | (Already in contact with technical support, but was asked to submit a review here as well.) Immunoblotting for ASCC3 from fresh cell extracts did not work. Included samples of ASCC3-KD (siRNA) experiment, but only unspecific bands. Tested 1:1000 and 1:500 dilution. Tested different protein extraction buffers and conditions, used fresh cells each time. Transfer of large proteins was confirmed. Sadly, for us this antibody did not work.
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