Various lysates were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 11529-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
human colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11529-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11529-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 11529-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11529-1-AP
IP result of anti-ASCC2 (IP:11529-1-AP, 4ug; Detection:11529-1-AP 1:1000) with HeLa cells lysate 2640ug.
IP result of anti-ASCC2 (IP:11529-1-AP, 4ug; Detection:11529-1-AP 1:1000) with HeLa cells lysate 2640ug.
IHC staining of human colon cancer using 11529-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11529-1-AP (ASCC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11529-1-AP (ASCC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11529-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (11529-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (11529-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HepG2 using 11529-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ASCC2 Polyclonal antibody (11529-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ASCC2 Polyclonal antibody (11529-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293T cells, human colon tissue, human brain tissue, HeLa cells, mouse colon tissue, mouse brain tissue, rat brain tissue
Positive IP detected in
HeLa cells
Positive IHC detected in
human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 11529-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11529-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11529-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 11529-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using 11529-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11529-1-AP (ASCC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 11529-1-AP
IP result of anti-ASCC2 (IP:11529-1-AP, 4ug; Detection:11529-1-AP 1:1000) with HeLa cells lysate 2640ug.
IF/ICC Figures
IF Staining of HepG2 using 11529-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (11529-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC (INTRA) Figures
FC experiment of HepG2 using 11529-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ASCC2 Polyclonal antibody (11529-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ASCC2 Polyclonal antibody
Catalog Number
11529-1-AP
Citations
3
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
